Bioreactor Unit for Photo Fermentation IKA

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Description

The HABITAT Photo Ferment control tower features LED panels essential for transforming you HABITAT research bioreactor into a photobioreactor. This system functions as a photo fermenter, suitable for specialised applications that integrate light into fermentation processes.

Photo fermenters enhance bioprocesses through controlled light and optimal conditions, crucial for specific biochemical reactions and microbial activities. For instance, they activate metabolic pathways in microorganisms to produce complex pharmaceuticals or boost the production of vitamins and beneficial metabolites in fermented food products.

The system includes two LED panels, the control unit with all connection options for gas supply, liquid addition, sensors, temperature control and a tablet for simple and clear operation of the fermenter.

Control and Monitoring

The intuitive software allows easy control and monitoring of the bioprocess and associated parameters via a 10.4inch tablet. User can select from batch, fed-batch and prefusion/continuous operating modes, catering to various cell culture techniques. The innovative Chaotic Mixing function enables efficient and homogeneous mixing when required. The control software provides clear calibration management, real-time data visualisation and complete process documentation, ensuring compliance with FDA CFR Part 11 requirements (optional).

Gas Supply

Precise and adjustable gassing is achieved through four individual built-in mass flow controllers for four separate gas lines (N2, O2, air and CO2), enabling flow rates of 0.01-2L/min. tailored to cell growth requirements. By using the integrated and freely customisable cascade control system, each gas flow can be automatically adjusted by the software at any time according to the ambient conditions in order to maintain target parameters.

Liquid Supply

Four integrated Watson-Marlow pumps with adjustable direction and speed off the highest precision and versatility in pumping in and out acid, base, anti-foam agents and feeding solutions for mammalian cell culture media management.

Sensors

HABITAT Bioreactor comes equipped with the latest technology sensors to precisely monitor and control critical bioprocess parameters. The following sensors are included in the standard sales vessel packages:

- pH

- DO (dissolved oxygen)

- temperature

- filling level

- foam

Optional sensors: CO2, Conductivity, Turbidity, Viable cell mass, Redox, Off-gas analysis and more.

PID Temperature Control

The integrated PID control ensures precise temperature regulation at all times, regardless of vessel type (single-wall or double-wall) or fill level. Single-wall glass vessels can be heated with a heating blanket, allowing for accurate temperature settings above room temperature. With the optional use of a cooling finger, it is also possible to achieve temperatures below room temperature. For double-walled vessels, in combination with an IKA thermostat, the temperature range can be freely adjusted between 5-80°C, independent of room conditions or heat generated by the culture itself.

Features and Benefits:

Compact space-saving design
Latest technology sensors for better insights and more control
Large screen tablet for easy operation
Intuitive, easy-to-use control software that provides simple initial setup and intuitive user assistance throughout the process
Four integrated Watson-Marlow pumps
High precision motor speed control
Cross platform connectivity: USB, PC, RS232, OPC-UA, ethernet, external signal output, external pump, single-use unit, thermostat
Data storage
Four built-in mass flow controllers for gas supply: N2, O2, air and CO2
Statue LED display: direct error display by indicator light

Note: Only a control tower and the separate available vessel package make up a functional unit.

Technical Data:

Controller	Fermenter
Operating mode	Timer, continuous, program operation
Display	Tablet PC
Operation	Touch screen
Data memory size	32 GB
Auto restart after power failure	Yes
Ethernet interface	Yes
Single use reactor interface	Yes
Thermostat interface	Yes
Ext. pimp interface	Yes
Gravimetric feeding	Yes
Exponential feeding	Yes
Biomass feeding	Yes, with Excell Sensor
OPC UA interface	Yes
Filter heater	Yes
LED light panel	Yes
Stirring motor	Yes
Calibration guide temperature	Yes
Calibration guide pH	Yes
Calibration guide pO2	Yes
pH - temperature compensation	Yes
Mode hose filling	Yes
Stirrer control mode	Off, manual, profile, Cascade-pO2 control, chaotic
Speed range	1 - 2200 rpm
Speed deviation >= 100 rpm	±2 %
Speed deviation <= 100 rpm	±5 rpm
Speed display	TFT
Reversible direction of rotation	Yes
Timer	Yes
Timer display	TFT
Time setting range	1 - 120 min
Heating blanket	Yes
Heat output	250W
Heat control	TFT
Temperature control	PID
Working temperature	5 - 80°C
Connection for ext. temperature sensor	PT1000
Temperature display	Yes
Temperature measuring range	0 - 100°C
Temperature measuring resolution	0.1K
Temperature control accuracy	±0.2°C
Temperature limit min.	0°C
Temperature limit max.	110°C
pH measurement	Yes
pH control mode	Off, auto, profile
pH value display	TFT
pH measuring range	0 - 14 pH
pH measuring resolution	0.01 pH
Accuracy of pH measurement	±0.02 pH
Set resolution pH value	0.01 pH
pH control accuracy	±0.05 pH
Connection for pO2 sensor	Yes
pO2 control mode	Off, manual, auto, profile
Measurement range pO2 min.	0 % sat
Measurement range pO2 max.	200 % sat
Resolution pO2 measurement	0.01 % sat
Accuracy of pO2 measurement	±0.3 % sat
pO2 control accuracy	0.3 % sat
Exhaust Gas Analyser	Yes, with Blue Vary Sensor
OUR, CER, RQ calculation	Yes, with Blue Vary Sensor
RQ feeding	Yes, with Blue Vary Sensor
Digital pH sensor	Yes, with EasyFerm Bio Arc Sensor
Measuring range min. digital pH	0 pH
Measuring range max. digital pH	14 pH
Accuracy digital pH	±0.02 pH
Digital DO sensor	Yes, with OxyFerm FDA Arc
Measuring range min. digital DO	0 % sat
Measuring range max. digital DO	200 % sat
Accuracy digital DO	±2 %
Optical DO sensor	Yes, with VisiFerm RS485-ECS
Measuring range min. optical DO	0 % sat
Measuring range max. optical DO	300 % sat
Accuracy optical DO	±1 %
Biomass sensor	Yes, with CGQ BioR Sensor
Measuring range min. biomass	0.5 OD
Measuring range max. biomass	50 OD
Digital Redox / ORP sensor	Yes, with EasyFerm Plus ORP Arc
Measuring range min. digital ORP	-1500 mV
Measuring range max. digital ORP	1500 mV
Digital biomass sensor	Yes, with Excell Sensor
Measuring range min. digital biomass	0 AU
Measuring range max. digital biomass	6 AU
Accuracy digital biomass	±1 %
Viable Cell Density Sensors	Yes, with Incyte Sensor
Measuring range min. cell density	5 x 10 ^ 5 cells/ml
Measuring range max. cell density	8 x 10 ^ 9 cells/ml
Accuracy cell density	±1 %
Gas in connections (air, O2, N2, CO2)	6mm
Gas out connections (air, O2, N2, CO2)	6mm
Aeration mode	Continuous, automatic
Display flowrate	l/min, cc/min
Input pressure	200 -3000 mbar
Pressure out min.	200 mbar
Pressure out max.	1000 mbar
Flowrate min. (air, O2, N2, CO2)	10 cc/min
Flowrate max. (air, O2, N2, CO2)	2000 cc/min
Measuring and control accuracy of the gas control units	±2 % fsr
Peristaltic pump	Yes
Number of pump heads	4
Pump head function	Acid, base, antifoam, level
Pump head	Watson-Marlow 114DV
Number of tubes	4
Number of rollers	4
Flow rate min.	0.3 ml/min
Flow rate max.	270 ml/min
Flow rate control accuracy	±0.3 ml/min
Pump speed min.	33 rpm
Pump speed max.	400 rpm
Pump reversible direction of rotation	Yes
Pump speed display	TFT
Inner tube diameter min.	0.5 mm
Inner tube diameter max.	4.8 mm
Tube outer diameter min.	4.1 mm
Tube outer diameter max.	8.4 mm
Tube material	Silicone
Tube hardness	50 - 60 Shore A
Permissible ambient temperature	5 - 40 °C
Permissible relative humidity	80% (up to 31°C), decreasing linearly to max. 50 (@40°C)
Protection class according to DIN EN 60529	IP 20
Interface	RS232, USB-B, WiFi
Power input	300W
Power input max.	1100W
Power input standby	10W
Fuse	2 x T5.0A 250V
Dimensions	224 x 624 x 405mm